Products

iTC200 Isothermal Titration Calorimetry

The iTC200 system is the most sensitive isothermal titration calorimeter available.  It is designed to address the needs of today’s life science researchers – particularly those engaged in drug discovery and development.  With its reduced size and associated sample quantity requirements, the iTC200 is significantly faster than previous models.  Designed for ease-of-use, all functions of the iTC200 are operated through software to facilitate fast and accurate analyses.

The iTC200 utilizes a 200 µl cell versus the 1.4 ml cell in previous models.
The iTC200 utilizes a 200 µl cell versus the 1.4 ml cell in previous models.

Applications include:

  • Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.
  • Lead optimization.
  • Enzyme kinetics.
  • Assessment of the effect of molecular structure changes on binding mechanisms.
  • Assessment of biological activitiy.

Why iTC200?

  • More than just affinities:  Simultaneous determination of all binding parameters in a single experiment - information unobtainable from more limited binding assays.
  • Directly measure sub-millimolar to nanomolar binding constants (102 to 109 M-1).  Measure nanomolar to picomolar binding constants (109 to 1012 M-1) using the competitive binding technique.
  • Application versatility:  Investigate any biomolecular interaction with high sensitivity.  Experiments require only 200 µl of sample and as little as 10 µg of protein in the sample cell.
  • True in-solution technique: No labeling or immobilization required. No buffer restrictions. Easily handles turbid solutions.
  • Easy to use:  Includes user friendly experimental design wizards and easy filling and cleaning procedures.
  • Fast time to first result:  With fast equilibration times, up to two runs per hour can be easily accomplished.  Requires no assay development or dedicated user to achieve high quality results.
  • Automatable:  iTC200 can be upgraded to a fully automated version with a sample throughput of at least 50 samples per day with a capacity to process as many as 384 samples unattended.
  • Complete system:  No additional accessories to purchase.  No reagents are required.
At Rest
At Rest
Sample Loading
Sample Loading
Titration
Titration
Washing
Washing

iTC200 features:

  • Active cell volume ~200 µl.
  • Non-reactive Hastelloy® cells for excellent chemical resistance.
  • Fixed-in-place cells for reproducible ultrasensitive performance with low maintenance.
  • Precision liquid delivery system for accurate and reproducible injections
  • User-selectable mixing speeds to match sample conditions.
  • Three user selectable response times (U.S. Patent number 5,967,659) for application versatility.
  • Operating temperature range of 2ºC to 80ºC.
  • Peltier controlled for rapid temperature equilibration.
  • Includes the iTC200 Washing Module for easy cell and syringe filling and  cleaning.

Need more information?  Contact us.

iTC200
iTC200